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refolding

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refolding是什么意思
英式音标:[re'fəʊldɪŋ]英式读音
美式音标:[re'foʊldɪŋ]美式读音
词义:

释义

重褶皱作用;

用法:

权威例句

Protein refolding for industrial processes

Advances in refolding of protein produced in E

Advances in refolding of proteins produced in E. coli

Solubilization and refolding of bacterial inclusion body proteins

GroE facilitates refolding of citrate synthase by suppressing aggregation

Posttranslational quality control: folding, refolding, and degrading proteins.

Contact order, transition state placement and the refolding rates of single domain proteins.

HLA-A2-peptide complexes: refolding and crystallization of molecules expressed in Escherichia coli and complexed with single antigen...

The folding of an enzyme. IV. Structure of an intermediate in the refolding of barnase analysed by a protein engineering procedure.

A kinetic study of the competition between renaturation and aggregation during the refolding of denatured-reduced egg white lysozyme
造句:

1. The unfolding and refolding of bovine serum albumins and the existence of intermediates were studied by fluorescence phase diagram.

荧光相图法研究牛血清白蛋白变性和复性过程,捕捉部分折叠中间态。

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2. The mechanism of oxidative refolding of proteins was elucidated in more detail from the intensive and extensive studies in the past decades.

经过近几十年来广泛而深入的研究,蛋白质氧化重折叠的机制已得到相当详细的阐明。

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3. Calcium and zinc ions were required both in refolding and enzymatic activity, but high concentration of zinc inhibited the refolding and activity.

该蛋白的重折叠复性和酶活性需要钙锌离子,但高浓度的锌离子则抑制其重折叠复性和活性。

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4. Most of the proteins examined so far proceed oxidative refolding via multiple pathways rather than a single and specific pathway. This is consistent with the folding energy landscape theory.

在已研究过的蛋白质中,大多数蛋白质都是沿着多途径而非单一、特定的途径进行氧化重折叠,这与折叠能量景观学说是一致的。

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5. The experimental results of urea gradient electrophoresis show that there are two different refolding processes.

尿素浓度梯度电泳实验结果显示,存在两条不同的折叠途径。

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6. AIM: To form soluble HLA-G1-peptide complex by refolding in vitro, and study its immune function.

目的:体外折叠形成可溶性HLAG1抗原肽复合物,并研究其生物学功能。

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7. Strategies for decreasing the formation of inclusion bodies, isolation and resolution of inclusion bodies, and refolding of inclusion body proteins were included.

综述了减少包涵体形成、包涵体分离和溶解以及包涵体折叠复性的策略及其最新进展。

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8. In addition, in the presence of the artificialchaperones, lysozyme refolding could be realized at high yield and folding rate at alow GdmCl concentration.

此外,若有人工伴侣的存在,在较低浓度的盐酸胍条件下就可实现快速、高收率的蛋白质复性。

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9. These inclusion bodies should be washed with denaturation buffer firstly, then followed by denaturation and refolding in proper buffer to harvest the active proteins.

包涵体中富含表达的重组蛋白质,它们经分离、洗涤、变性溶解以及复性等过程才能得到具有生物活性的蛋白质。

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10. Then according to experiment design, the effects of rChIL-18 protein refolding yield at different densities were investigated by the systems of artificial chaperone at different densities.

然后按照试验设计,考察不同浓度组成的人工分子伴侣体系对不同浓度鸡IL-18重组蛋白复性率的影响。

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11. Investigations on protein refolding by size-exclusion chromatography (SEC) was carried out by using lysozyme as a model system.

以溶菌酶为模拟体系对体积排阻色谱法进行蛋白质折叠过程实验研究。

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12. The research into protein refolding techniques is of essential importance to the down stream processing of genetically engineered proteins.

蛋白质复性技术的研究对于基因工程重组蛋白质的生产具有重要意义。

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13. Objective The possibility of peripherin binding protein (PBP) to improve the refolding of denatured luciferase in vitro was investigated.

目的探讨感光受体外周蛋白结合蛋白(PBP)在体外是否有促进变性蛋白质复性的作用。

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14. The kinetic model was in good agreement with the experimental results. The mechanism of CAB refolding assisted by amides were analogous to that of GdmCl.

利用三级反应动力学方程拟和酰胺化合物辅助CAB复性的动力学过程。结果表明,模型计算值与实验数据能较好吻合。

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15. The possibility of peripherin binding protein (PBP) to improve the refolding of denatured luciferase in vitro was investigated.

探讨感光受体外周蛋白结合蛋白(PBP)在体外是否有促进变性蛋白质复性的作用。

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16. Conclusion The parameters of expression, purification and refolding of sCR1-SCR15-18 protein were optimized, which may pave a way for further studies.

结论优化了sCR1-SCR15-18蛋白的表达、纯化和复性的参数,所获结果为进一步动物体内保护实验研究奠定了基础。

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17. The results showed that the refolding efficiency of proteins denatured by GuHCl is the highest, and SDS is the lowest in the three denaturing agents.

盐酸胍变性的蛋白质复性后的质量回收率和活性回收率均高于尿素,SDS变性的蛋白质构象难以恢复,复性效果最差。

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18. Methods Based on the reactive principle of molecular chaperone, we adopted an artificial molecular chaperone-assisted refolding method for recombinant endostatin refolding.

方法根据人工分子伴侣对重组蛋白质的复性机理,采用人工分子伴侣方法应用于重组内抑素的复性。

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19. Liquid chromatography (LC) is one of the most effective way to separate and purify biopolymer, and also a powerful tool to be used for protein refolding.

高效液相色谱不仅用于生物大分子的分离,也是蛋白折叠研究的重要手段,已发展成为蛋白折叠液相色谱法。

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20. In this thesis, unfolding and refolding of human group IB and X phospholipase A2 have been studied.

本论文以人的IB和X型磷脂酶A2为研究对象,研究了蛋白质的去折叠和重折叠反应。

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21. The recent research progress of recombinant protein refolding was reviewed.

本文对近年来重组蛋白复性技术的研究进行了评述。

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22. Conclusion The refolding of sHLA-G1-peptide complex can be successfully generated with Prokaryotic expressing and Refolding in vitro.

结论通过原核表达、体外折叠能够获得大量可溶性HLA-G1-肽复合物。

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23. Recently, with the development of studying in mechanism of protein refolding, some new strategies were reported to solve this problem.

近年来,随着对包涵体体外复性机制的深入研究,从包涵体中复性重组蛋白质已经有了很多的策略和方法。

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24. AIM: To improve the refolding efficiency of soluble HLA-A2-peptide complex in vitro.

目的:提高可溶性HLA-A2肽复合物体外折叠效率。

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25. In this chapter solute retention mechanism in LC, protein refolding mechanism and new developments in protein refolding kinetics were reviewed. It contains 61 references.

本章对色谱中的保留机理及理论、蛋白质液相色谱复性机理及蛋白折叠动力学理论模型研究进展进行了综述,共包括文献61篇。

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26. The interface of liquid-solid in liquid chromatography (LC) gives main contribution to protein refolding, so the impacts of the solute adsorption in the liquid-solid systems were investigated firstly.

由于液相色谱中固定相与流动相之间的界面,即液-固界面对蛋白折叠起着至关重要的作用。

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27. To increase ratio of correctly folded recombinant proteins, we purified the protein disulfide isomerase (PDI) from bovine liver, and studied the effects of enzyme catalyzed refolding process.

自牛肝中纯化了蛋白二硫键异构酶(PDI),并对重组蛋白的酶促折叠过程进行了探讨。

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28. Protein refolding by dilution is the basis of research on other method of renaturation.

稀释复性法是研究其他方法的基础。

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29. Protein refolding by dilution is the basis of research on other method of renaturation.

稀释复性法是研究其他方法的基础。

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30. refolding of cell

容器 的折叠

-- 来源 -- 英汉 - 翻译参考[网络]

31. The aggregate of egg white lysozyme molecules formed during the refolding procedure of denatured-reduced egg white lysozymes was analyzed by protein electrophoreses and high-performance size-exclusion chromatography.

本文利用蛋白电泳和高效凝胶排阻层析法分析了还原脉变性蛋白溶菌酶稀释复性过程中的集聚体。

-- 来源 -- www.21cnchem.com

32. Cloth centred paper: Paper with a linen centre, often used for maps when much refolding is anticipated.

布心纸:麻布夹心的纸,常作地图用,以应付使用时的多次摺合。

-- 来源 -- 英汉 - 翻译参考[网络]

refolding

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